ARG66596
anti-MRPS12 antibody
anti-MRPS12 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MRPS12 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MRPS12 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 40-120 of Human MRPS12. |
| Conjugation | Un-conjugated |
| Alternate Names | RPSM12; RPS12; 28S ribosomal protein S12, mitochondrial; MT-RPS12; RPMS12; MRP-S12; S12mt; MPR-S12 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15235 Human 28S ribosomal protein S12, mitochondrial |
|---|---|
| Gene Symbol | MRPS12 |
| Gene Full Name | mitochondrial ribosomal protein S12 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 15 kDa |
Images (1) Click the Picture to Zoom In
-
ARG66596 anti-MRPS12 antibody WB image
Western blot: SH-SY5Y cell lysate stained with ARG66596 anti-MRPS12 antibody at 1:1000 dilution.
