ARG65512
anti-MPO / Myeloperoxidase antibody [MPO421-8B2]
anti-MPO / Myeloperoxidase antibody [MPO421-8B2] for ELISA,Flow cytometry and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [MPO421-8B2] recognizes MPO / Myeloperoxidase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS |
| Specificity | The mouse monoclonal antibody MPO4218B2 recognizes human myeloperoxidase, a heme protein present in myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MPO421-8B2 |
| Isotype | IgG1 |
| Target Name | MPO / Myeloperoxidase |
| Antigen Species | Human |
| Immunogen | Human myeloperoxidase |
| Conjugation | Un-conjugated |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | FACS: Intracellular staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPO |
| Gene Full Name | myeloperoxidase |
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Highlight | Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| Calculated MW | 84 kDa |
Images (2) Click the Picture to Zoom In
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ARG65512 anti-MPO / Myeloperoxidase antibody [MPO421-8B2] FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG65512 anti-MPO / Myeloperoxidase antibody [MPO421-8B2] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65512 anti-MPO / Myeloperoxidase antibody [MPO421-8B2] FACS image
Flow Cytometry: Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65512 anti-MPO / Myeloperoxidase antibody [MPO421-8B2] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
