ARG43748
anti-MPO / Myeloperoxidase antibody [MPO/33R]
anti-MPO / Myeloperoxidase antibody [MPO/33R] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [MPO/33R] recognizes MPO / Myeloperoxidase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | MPO/33R |
| Isotype | IgG |
| Target Name | MPO / Myeloperoxidase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 150-250 of Human MPO / Myeloperoxidase. |
| Conjugation | Un-conjugated |
| Protein Full Name | Myeloperoxidase |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human spleen | ||||||
| Observed Size | 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A/G affinity. |
| Buffer | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPO |
| Gene Full Name | myeloperoxidase |
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| Calculated MW | 60 kDa, 84 kDa, 89 kDa |
Images (2) Click the Picture to Zoom In
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ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R].
Negative control inset: PBS instead of primary antibody to control for secondary binding.
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ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R] WB image
Western blot: Human spleen lysate stained with ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R].
