ARG43747
anti-MPO / Myeloperoxidase antibody
anti-MPO / Myeloperoxidase antibody for Flow cytometry,Western blot and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MPO / Myeloperoxidase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MPO / Myeloperoxidase |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to S406-S745 of Human Myeloperoxidase / MPO. |
| Conjugation | Un-conjugated |
| Protein Full Name | Myeloperoxidase |
| Alternate Names | MPO; Myeloperoxidase; EC 1.11.2.2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HL-60 | ||||||
| Observed Size | 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPO |
| Gene Full Name | myeloperoxidase |
| Background | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Function | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30325 Inflammatory Cell Antibody Panel Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| Calculated MW | 60 kDa, 84 kDa, 89 kDa |
Images (2) Click the Picture to Zoom In
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ARG43747 anti-MPO / Myeloperoxidase antibody WB image
Western blot: 30 µg of HL-60 lysate under reducing conditions stained with ARG43747 anti-MPO / Myeloperoxidase antibody at 0.5 µg/ml, overnight at 4°C.
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ARG43747 anti-MPO / Myeloperoxidase antibody FACS image
Flow Cytometry: Human HL-60 cells were blocked with 10% normal goat serum and then stained with ARG43747 anti-MPO / Myeloperoxidase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
