ARG63446

anti-MPG antibody

anti-MPG antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes MPG
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MPG
Antigen Species Human
Immunogen C-SVVDRVAEQDTQA
Conjugation Un-conjugated
Alternate Names AAG; APNG; anpg; 3-alkyladenine DNA glycosylase; N-methylpurine-DNA glycosylase; PIG16; PIG11; ADPG; MDG; 3-methyladenine DNA glycosidase; CRA36.1; DNA-3-methyladenine glycosylase; EC 3.2.2.21; Mid1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4350 Human MPG

Swiss-port # P29372 Human DNA-3-methyladenine glycosylase

Gene Symbol MPG
Gene Full Name N-methylpurine-DNA glycosylase
Function Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 33 kDa

Images (2) Click the Picture to Zoom In

  • ARG63446 anti-MPG antibody WB image

    Western Blot: HEK293 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63446 anti-MPG antibody at 1 µg/ml dilution.

  • ARG63446 anti-MPG antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63446 anti-MPG antibody at 5 µg/ml dilution followed by AP-staining.