ARG44180
anti-MNAT1 antibody
anti-MNAT1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MNAT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MNAT1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human MNAT1 |
| Conjugation | Un-conjugated |
| Protein Full Name | CDK-activating kinase assembly factor MAT1 |
| Alternate Names | MNAT1; MNAT1 Component Of CDK Activating Kinase; RNF66; MAT1; MNAT CDK-Activating Kinase Assembly Factor 1; MNAT1, CDK Activating Kinase Assembly Factor; CDK-Activating Kinase Assembly Factor MAT1; Menage A Trois 1 (CAK Assembly Factor); CDK7/Cyclin-H Assembly Factor; RING Finger Protein MAT1; RING Finger Protein 66; CAP35; Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis); Menage A Trois-Like Protein 1 Cyclin H Assembly Factor; Menage A Trois Homolog 1, Cyclin H Assembly Factor; CDK-Activating Kinase Assembly Factor; Cyclin G1 Interacting Protein; Cyclin-G1-Interacting Protein; Menage A Trois; TFB3; P35; P36 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51948 Human CDK-activating kinase assembly factor MAT1 Swiss-port # P51949 Mouse CDK-activating kinase assembly factor MAT1 |
|---|---|
| Gene Symbol | MNAT1 |
| Gene Full Name | Mnat1 Component Of Cdk Activating Kinase |
| Background | The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. |
| Function | Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. |
| Cellular Localization | Nucleus |
| Calculated MW | 36 kDa |
| PTM | Acetylation, Phosphoprotein |
Images (5) Click the Picture to Zoom In
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ARG44180 anti-MNAT1 antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG44180 anti-MNAT1 antibody at 5 μg/mL dilution.
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ARG44180 anti-MNAT1 antibody WB image
Western blot: A431, HeLa and A375 stained with ARG44180 anti-MNAT1 antibody at 0.25 μg/mL dilution.
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ARG44180 anti-MNAT1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG44180 anti-MNAT1 antibody at 1 μg/1x10^6 cells dilution.
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ARG44180 anti-MNAT1 antibody WB image
Western blot: Rat testis and RH35 stained with ARG44180 anti-MNAT1 antibody at 0.25 μg/mL dilution.
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ARG44180 anti-MNAT1 antibody WB image
Western blot: Mouse testis and NIH/3T3 stained with ARG44180 anti-MNAT1 antibody at 0.25 μg/mL dilution.
