ARG66424
anti-MMP7 (active) antibody [SQab18126]
anti-MMP7 (active) antibody [SQab18126] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SQab18126] recognizes MMP7 (active) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18126 |
| Isotype | IgG2a, kappa |
| Target Name | MMP7 (active) |
| Antigen Species | Human |
| Immunogen | Recombinant Human MMP7 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP7 |
| Gene Full Name | matrix metallopeptidase 7 |
| Background | MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016] |
| Function | MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Calculated MW | 30 kDa |
Images (4) Click the Picture to Zoom In
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ARG66424 anti-MMP7 (active) antibody [SQab18126] ICC/IF image
Immunofluorescence: A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66424 anti-MMP7 (active) antibody [SQab18126] (green) at 1:200 dilution and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66424 anti-MMP7 (active) antibody [SQab18126] FACS image
Flow Cytometry: A549 cells were stained with ARG66424 anti-MMP7 (active) antibody [SQab18126] at 2 µg/ml (red) and without antibody control (black).
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ARG66424 anti-MMP7 (active) antibody [SQab18126] WB image
Western blot: 50 ng of Recombinant Pro-MMP7, 50 ng of Recombinant MMP7 (active), 30 µg of 293T, 30 µg of LPS treated 293T, 30 µg of MMP7 transfected 293T and 30 µg of LPS treated, MMP7 transfected 293T cell lysates stained with ARG66424 anti-MMP7 (active) antibody [SQab18126] at 1:5000 dilution. The expression of Actin was as the internal control.
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ARG66424 anti-MMP7 (active) antibody [SQab18126] ELISA image
ELISA: Titration curve of ARG66424 anti-MMP7 (active) antibody [SQab18126]. Red: Active MMP7; Green: Pro-MMP7; Black: BSA (negative control).
