Overview

Product Description	Rabbit Polyclonal antibody recognizes MMP2
Tested Reactivity	Hu
Tested Application	ELISA, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MMP2
Antigen Species	Human
Immunogen	Human MMP2 recombinant protein
Expression System	E.coli
Conjugation	Un-conjugated
Protein Full Name	72 kDa type IV collagenase
Alternate Names	MMP2; Matrix Metallopeptidase 2; TBE-1; CLG4A; 72 KDa Type IV Collagenase; Matrix Metalloproteinase-2; EC 3.4.24.24; MMP-2; CLG4; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase) ; Matrix Metalloproteinase-II; Collagenase Type IV-A; Neutrophil Gelatinase; 72 KDa Gelatinase; Gelatinase A; EC 3.4.24; MMP-II; MONA

Application Instructions

Application Suggestion	Tested Application Dilution ELISA 0.1-0.5 µg/ml WB 0.25-0.5 µg/ml
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer	4% Trehalose
Concentration	0.5 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links	GeneID: 4313 Human MMP2 Swiss-port # P08253 Human 72 kDa type IV collagenase
Gene Symbol	MMP2
Gene Full Name	Matrix Metallopeptidase 2
Background	This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Function	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
Cellular Localization	Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted
Calculated MW	74 kDa
PTM	Autocatalytic cleavage, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

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