ARG41614
anti-MMP13 antibody
anti-MMP13 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MMP13 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MMP13 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 262-471 of Human MMP13 (NP_002418.1). |
| Conjugation | Un-conjugated |
| Alternate Names | MMP13; Matrix Metallopeptidase 13; Collagenase 3; CLG3; Matrix Metalloproteinase 13 (Collagenase 3); MMP-13; Matrix Metalloproteinase-13; EC 3.4.24.35; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.65; EC 3.4.24.; EC 3.4.24.7; EC 3.4.24; MANDP1; MDST |
Application Instructions
| Predict Reactivity Note | Rat | ||||
|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse intestine | ||||
| Observed Size | ~ 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP13 |
| Gene Full Name | matrix metallopeptidase 13 |
| Background | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016] |
| Function | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. [UniProt] |
| Cellular Localization | Extracellular matrix, Secreted. [UniProt] |
| Calculated MW | 54 kDa |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41614 anti-MMP13 antibody WB image
Western blot: 25 µg of Mouse intestine lysate stained with ARG41614 anti-MMP13 antibody at 1:1000 dilution.
