Overview

Product Description	Rabbit Polyclonal antibody recognizes MMP12
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MMP12
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human MMP12.
Conjugation	Un-conjugated
Alternate Names	ME; EC 3.4.24.65; Macrophage metalloelastase; MMP-12; hME; MME; HME; Matrix metalloproteinase-12; Macrophage elastase

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 IP 1:30 WB 1:1000 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	WI-38

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 117033 Rat MMP12 GeneID: 17381 Mouse MMP12 GeneID: 4321 Human MMP12 Swiss-port # P34960 Mouse Macrophage metalloelastase Swiss-port # P39900 Human Macrophage metalloelastase Swiss-port # Q63341 Rat Macrophage metalloelastase More
Gene Symbol	MMP12
Gene Full Name	matrix metallopeptidase 12
Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Function	May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. [UniProt]
Cellular Localization	Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW	54 kDa

Images (1) Click the Picture to Zoom In