ARG59021
anti-MMP10 antibody
anti-MMP10 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MMP10 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MMP10 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to Y239-C476 of Human MMP10. |
| Conjugation | Un-conjugated |
| Alternate Names | SL-2; STMY2; Transin-2; EC 3.4.24.22; Matrix metalloproteinase-10; MMP-10; Stromelysin-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP10 |
| Gene Full Name | matrix metallopeptidase 10 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| Function | Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Calculated MW | 54 kDa |
Images (5) Click the Picture to Zoom In
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ARG59021 anti-MMP10 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG59021 anti-MMP10 antibody.
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ARG59021 anti-MMP10 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then staind with ARG59021 anti-MMP10 antibody at 1 μg/ml, overnight at 4°C.
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ARG59021 anti-MMP10 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat cardiac muscle and Mouse cardiac muscle lysates stained with ARG59021 anti-MMP10 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59021 anti-MMP10 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59021 anti-MMP10 antibody at 1 μg/ml, overnight at 4°C.
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ARG59021 anti-MMP10 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta lysate stained with ARG59021 anti-MMP10 antibody at 0.5 µg/ml, overnight at 4°C.
