ARG43682

anti-MLLT10 / AF10 antibody

anti-MLLT10 / AF10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MLLT10 / AF10
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_004632.1; NP_001009569.1).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MLLT10 / AF10
Antigen Species Human
Immunogen Synthetic peptide of Human MLLT10 / AF10.
Conjugation Un-conjugated
Protein Full Name Protein AF-10
Alternate Names Protein AF-10; AF10; ALL1-fused gene from chromosome 10 protein; MLLT10 histone lysine methyltransferase DOT1L cofactor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 130 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Concentration Batch dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8028 Human MLLT10

Swiss-port # P55197 Human Protein AF-10

Gene Symbol MLLT10
Gene Full Name MLLT10 histone lysine methyltransferase DOT1L cofactor
Background This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Function Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (PubMed:26439302). [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
Calculated MW 113 kDa
PTM Isopeptide bond; Phosphoprotein; Ubl conjugation