ARG66188
anti-MIF antibody
anti-MIF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MIF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | The antibody detects endogenous MIF protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MIF |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the Internal region of Human MIF. |
| Conjugation | Un-conjugated |
| Alternate Names | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MIF |
| Gene Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Background | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt] |
| Calculated MW | 12 kDa |
Images (27) Click the Picture to Zoom In
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat lung tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody WB image
Western blot: HepG2 cell lysate stained with ARG66188 anti-MIF antibody at 1:500 dilution.
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat lung tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat spleen tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody IHC image
Immunohistochemistry: Rat spleen tissue stained with ARG66188 anti-MIF antibody (red) at 1:200 dilution (4°C, overnight).
Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus cancer tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Tonsil tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach cancer tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat heart tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse heart tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
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ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
-
ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
-
ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
-
ARG66188 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG66188 anti-MIF antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
Negative control was used by secondary antibody only.
