ARG63789
anti-MIF antibody
anti-MIF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MIF |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MIF |
| Antigen Species | Human |
| Immunogen | C-NAANVGWNNSTFA |
| Conjugation | Un-conjugated |
| Alternate Names | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14174 Human Macrophage migration inhibitory factor |
|---|---|
| Background | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| Research Area | Immune System antibody |
| Calculated MW | 12 kDa |
Images (3) Click the Picture to Zoom In
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ARG63789 anti-MIF antibody WB image
Western Blot: Daudi (A) and Jurkat (B) lysate (35 µg protein in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution.
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ARG63789 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63789 anti-MIF antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63789 anti-MIF antibody WB image
Western blot: 35 µg of Human thymus lysate (in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
