ARG40481
anti-MIF antibody
anti-MIF antibody for Immunocytochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MIF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MIF |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to P2-A115 Human MIF. |
| Conjugation | Un-conjugated |
| Alternate Names | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14174 Human Macrophage migration inhibitory factor |
|---|---|
| Gene Symbol | MIF |
| Gene Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Background | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt] |
| Cellular Localization | Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. [UniProt] |
| Calculated MW | 12 kDa |
Images (3) Click the Picture to Zoom In
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ARG40481 anti-MIF antibody ICC image
Immunocytochemistry: Jurkat cells stained with ARG40481 anti-MIF antibody.
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ARG40481 anti-MIF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG40481 anti-MIF antibody.
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ARG40481 anti-MIF antibody WB image
Western blot: 40 µg of U87, Jurkat, HUT, A549 and HepG2 whole cell lysates stained with ARG40481 anti-MIF antibody at 0.5 µg/ml dilution.
