ARG56922
anti-MIF antibody [4E4]
anti-MIF antibody [4E4] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4E4] recognizes MIF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4E4 |
| Isotype | IgG1, kappa |
| Target Name | MIF |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-114 of Human MIF. |
| Conjugation | Un-conjugated |
| Alternate Names | Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14174 Human Macrophage migration inhibitory factor |
|---|---|
| Gene Symbol | MIF |
| Gene Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Background | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt] |
| Calculated MW | 12 kDa |
Images (4) Click the Picture to Zoom In
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ARG56922 anti-MIF antibody [4E4] ICC/IF image
Immunoflorescense: Balb/3T3 cell line stained with ARG56922 anti-MIF antibody [4E4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56922 anti-MIF antibody [4E4] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) THP-1 cell lysate, 3) HeLa cell lysate, and 4) U937 cell lysate stained with ARG56922 anti-MIF antibody [4E4] at 1:1000.
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ARG56922 anti-MIF antibody [4E4] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56922 anti-MIF antibody [4E4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56922 anti-MIF antibody [4E4] WB image
Western blot: HL-60 cell lysate stained with ARG56922 anti-MIF antibody [4E4] at 1:1000.
