ARG66109

anti-MIA antibody (Biotin)

anti-MIA antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes MIA
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MIA
Antigen Species Human
Immunogen E. coli derived recombinant Human MIA.
(MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ)
Conjugation Biotin
Alternate Names CD-RAP; Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66108 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8190 Human MIA

Swiss-port # Q16674 Human Melanoma-derived growth regulatory protein

Gene Symbol MIA
Gene Full Name melanoma inhibitory activity
Function Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProt]
Calculated MW 15 kDa
PTM May possess two intramolecular disulfide bonds.

Images (4) Click the Picture to Zoom In

  • ARG66109 anti-MIA antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human MIA stained with ARG66109 anti-MIA antibody (Biotin), under non-reducing conditions.

  • ARG66109 anti-MIA antibody (Biotin) standard curve image

    Direct ELISA: ARG66109 anti-MIA antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66109 anti-MIA antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human MIA stained with ARG66109 anti-MIA antibody (Biotin), under reducing conditions.

  • ARG66109 anti-MIA antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66109 anti-MIA antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66108 anti-MIA antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.