ARG65479
anti-MHC Class II antibody [M5/114] (azide free)
anti-MHC Class II antibody [M5/114] (azide free) for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse
Immune System antibody
Overview
| Product Description | Azide free Rat Monoclonal antibody [M5/114] recognizes MHC Class II |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, FuncSt, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | M5/114 |
| Isotype | IgG2b |
| Target Name | MHC Class II |
| Antigen Species | Mouse |
| Immunogen | Activated C57BL/6 mouse spleen cells. |
| Conjugation | Un-conjugated |
| Alternate Names | HLA-DRB; HLA class II histocompatibility antigen, DRB1-3 chain; SS1; MHC class II antigen DRB1*3; HLA-DR1B; DRw10; Clone P2-beta-3; DRB1 |
Application Instructions
| Application Suggestion |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | Functional Application: Blocking of T cell proliferative responses * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | H2-D1 |
|---|---|
| Gene Full Name | histocompatibility 2, D region locus 1 |
| Background | MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. |
| Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 30 kDa |
| PTM | Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II. |
Images (1) Click the Picture to Zoom In
-
ARG65479 anti-MHC Class II antibody [M5/114] (azide free) FACS image
Flow Cytometry: Murine splenocytes stained with ARG65479 anti-MHC Class II antibody [M5/114] (azide free), followed by APC-conjugated Goat anti-Rat antibody.
