ARG21080
anti-MHC Class I H2 Dd antibody [34-5-8S]
anti-MHC Class I H2 Dd antibody [34-5-8S] for Blocking,ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Purification and Mouse
Overview
| Product Description | Mouse Monoclonal antibody [34-5-8S] recognizes MHC Class I H2 Dd |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | BL, ELISA, FACS, ICC/IF, IP, Puri |
| Specificity | The clone 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of α1 and α2 chains when complexed with β2-microglobulin. The antibody does not react with H-2Dd α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 34-5-8S |
| Isotype | IgG2a, kappa |
| Target Name | MHC Class I H2 Dd |
| Antigen Species | Mouse |
| Immunogen | BDF1 mouse splenocytes |
| Conjugation | Un-conjugated |
| Alternate Names | H-2 class I histocompatibility antigen, D-B alpha chain; H2-D; B; H-2D |
Application Instructions
| Application Suggestion |
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| Application Note | Blocking: Antibody clone [34-5-8S] recognizing distinct D^d alpha1/alpha2 domain blocks EL4 adhesion to affinity-purified D^d. PMID: 8113669 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01899 Mouse H-2 class I histocompatibility antigen, D-B alpha chain |
|---|---|
| Gene Symbol | H2-D1 |
| Gene Full Name | histocompatibility 2, D region locus 1 |
| Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| Calculated MW | 41 kDa |
