ARG62544

anti-MGMT antibody [MT 3.1]

anti-MGMT antibody [MT 3.1] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [MT 3.1] recognizes MGMT
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone MT 3.1
Isotype IgG1
Target Name MGMT
Antigen Species Human
Immunogen Purified recombinant human MGMT protein
Conjugation Un-conjugated
Alternate Names O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
IHC-P1:10 - 1:20
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Raji cells. Colon CA

Properties

Form Liquid
Purification Protein G purified
Buffer 1X PBS buffer with < 0.1% sodium azide.
Preservative < 0.1% sodium azide.
Concentration 2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4255 Human MGMT

Swiss-port # P16455 Human Methylated-DNA--protein-cysteine methyltransferase

Gene Symbol MGMT
Gene Full Name O-6-methylguanine-DNA methyltransferase
Background Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
Function MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
Calculated MW 22 kDa

Clone References

Infratentorial low-grade oligoastrocytoma with aggressive clinical behavior in an adult: a case report with genetic characterization.

IHC / Human

Sano K et al.
Brain Tumor Pathol.,  (2013)

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Clinicopathological features of human brainstem gliomas.

IHC / Human

Oka H et al.
Brain Tumor Pathol.,  (2013)

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MGMT promoter hypermethylation and its associations with genetic alterations in a series of 350 brain tumors.

WB / Mouse

Mellai M et al.
J Neurooncol.,  (2012)

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Expression of O⁶-methylguanine-DNA methyltransferase in childhood medulloblastoma.

WB / Human

Faoro D et al.
J Neurooncol.,  (2011)

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Methylguanine DNA methyltransferase-mediated drug resistance-based selective enrichment and engraftment of transplanted stem cells in skeletal muscle.

ICC/IF / Human

Lee AS et al.
Stem Cells.,  (2009)

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