ARG40462
anti-MGAT5 antibody
anti-MGAT5 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MGAT5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MGAT5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 627-741 of Human MGAT5 (NP_002401.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.4.1.155; Mannoside acetylglucosaminyltransferase 5; GlcNAc-T V; GNT-VA; GNT-V; Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; N-acetylglucosaminyl-transferase V; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Jurkat | ||||
| Observed Size | 115 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q09328 Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Swiss-port # Q8R4G6 Mouse Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A |
|---|---|
| Gene Symbol | MGAT5 |
| Gene Full Name | mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase |
| Background | The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011] |
| Function | Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 85 kDa |
Images (1) Click the Picture to Zoom In
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ARG40462 anti-MGAT5 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG40462 anti-MGAT5 antibody at 1:1000 dilution.
