ARG44143
anti-MGAT4B antibody
anti-MGAT4B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal recognizes MGAT4B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MGAT4B |
| Antigen Species | Human |
| Immunogen | Human MGAT4B recombinant protein (Position: Q31-D548). |
| Conjugation | Un-conjugated |
| Alternate Names | MGAT4B; Alpha-1,3-Mannosyl-Glycoprotein 4-Beta-N-Acetylglucosaminyltransferase B; UDP-N-Acetylglucosamine: Alpha-1,3-D-Mannoside Beta-1,4-N-Acetylglucosaminyltransferase IVb; Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,4-N-Acetylglucosaminyltransferase, Isoenzyme B; Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,4-N-Acetylglucosaminyltransferase, Isozyme B; N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase IVb; N-Acetylglucosaminyltransferase IVb; GlcNAc-T IVb; EC 2.4.1.145; GnT-Ivb; UDP-N-Acetylglucosamine: Alpha-1,3-D-Mannoside Beta-1,4-N-Acetylglucosaminyltransferase IV; Alpha-1,3-Mannosyl-Glycoprotein Beta-1,4-N-Acetylglucosaminyltransferase; Aminyltransferase; GNT-IVB; GnT-IVb; GNT-IV |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q812F8 Mouse Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B Swiss-port # Q9UQ53 Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B |
|---|---|
| Gene Symbol | MGAT4B |
| Gene Full Name | Alpha-1,3-Mannosyl-Glycoprotein 4-Beta-N-Acetylglucosaminyltransferase B |
| Background | This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. |
| Function | Glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains. |
| Cellular Localization | Golgi apparatus, Membrane |
| Calculated MW | 63 kDa |
| PTM | Glycoprotein |
Images (4) Click the Picture to Zoom In
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ARG44143 anti-MGAT4B antibody IHC-P image
Immunohistochemistry: Human glioma stained with ARG44143 anti-MGAT4B antibody at 2 μg/ml dilution.
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ARG44143 anti-MGAT4B antibody WB image
Western blot: HepG2, RT4 and A431 stained with ARG44143 anti-MGAT4B antibody at 0.5 μg/ml dilution.
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ARG44143 anti-MGAT4B antibody WB image
Western blot: Rat kidney stained with ARG44143 anti-MGAT4B antibody at 0.5 μg/ml dilution.
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ARG44143 anti-MGAT4B antibody WB image
Western blot: Mouse kidney stained with ARG44143 anti-MGAT4B antibody at 0.5 μg/ml dilution.
