ARG44141

anti-MGAT2 antibody

anti-MGAT2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal recognizes MGAT2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MGAT2
Antigen Species Human
Immunogen Human MGAT2 recombinant protein (Position: Q81-K427).
Conjugation Un-conjugated
Alternate Names MGAT2; Alpha-1,6-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase; GNT-II; Mannosyl (Alpha-1,6-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase; N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase II; Beta-1,2-N-Acetylglucosaminyltransferase II; Mannoside Acetylglucosaminyltransferase 2; EC 2.4.1.143; GlcNAc-T II; UDP-N-Acetylglucosamine:Alpha-6-D-Mannoside Beta-1,2-N-Acetylglucosaminyltransferase II; GLCNACTII; CDG2A; CDGS2; GNT2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2 - 5 µg/ml
WB0.25 - 0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 217664 Mouse MGAT2

GeneID: 4247 Human MGAT2

GeneID: 94273 Rat MGAT2

Gene Symbol MGAT2
Gene Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Background The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Function Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. [UniProt]
Cellular Localization Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt]
Calculated MW 52 kDa

Images (6) Click the Picture to Zoom In

  • ARG44141 anti-MGAT2 antibody IHC-P image

    Immunohistochemistry: Human large B-cell lymphoma stained with ARG44141 anti-MGAT2 antibody at 2 μg/ml dilution.

  • ARG44141 anti-MGAT2 antibody WB image

    Western blot: SiHa, HepG2 and RT4 stained with ARG44141 anti-MGAT2 antibody at 0.5 μg/ml dilution.

  • ARG44141 anti-MGAT2 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44141 anti-MGAT2 antibody at 1 µg/10^6 cells dilution.

  • ARG44141 anti-MGAT2 antibody WB image

    Western blot: Rat live  stained with ARG44141 anti-MGAT2 antibody at 0.5 μg/ml dilution.

  • ARG44141 anti-MGAT2 antibody WB image

    Western blot: Mouse live  stained with ARG44141 anti-MGAT2 antibody at 0.5 μg/ml dilution.

  • ARG44141 anti-MGAT2 antibody IHC-P image

    Immunohistochemistry: Human rectal cancer stained with ARG44141 anti-MGAT2 antibody at 2 μg/ml dilution.