ARG40443
anti-MGAT1 antibody
anti-MGAT1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MGAT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MGAT1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 156-445 of Human MGAT1 (NP_001108092.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; GLCNAC-TI; GNT-I; GNT-1; GLCT1; GLYT1; MGAT; GlcNAc-T I; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I; EC 2.4.1.101 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse liver | ||||||
| Observed Size | 57 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MGAT1 |
| Gene Full Name | mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase |
| Background | There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 51 kDa |
Images (2) Click the Picture to Zoom In
-
ARG40443 anti-MGAT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver injury stained with ARG40443 anti-MGAT1 antibody at 1:100 dilution.
-
ARG40443 anti-MGAT1 antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG40443 anti-MGAT1 antibody at 1:1000 dilution.
