ARG63070
anti-MFGE8 / Lactadherin antibody [MFG-06]
anti-MFGE8 / Lactadherin antibody [MFG-06] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [MFG-06] recognizes MFGE8 / Lactadherin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | The clone MFG-06 reacts with a 40-45 kDa glycoprotein in human milk fat globule membrane preparation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MFG-06 |
| Isotype | IgG1 |
| Target Name | MFGE8 / Lactadherin |
| Antigen Species | Human |
| Immunogen | Human milk fat globule membrane preparation. |
| Conjugation | Un-conjugated |
| Alternate Names | SPAG10; MFGM; HsT19888; HMFG; Milk fat globule-EGF factor 8; MFG-E8; BA46; hP47; EDIL1; Lactadherin; OAcGD3S; Breast epithelial antigen BA46; SED1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Strongly positive on human epithelia-based structures. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MFGE8 |
| Gene Full Name | milk fat globule-EGF factor 8 protein |
| Background | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 43 kDa |
| PTM | Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. |
Clone References
Differentiation patterns of testicular germ-cell tumours as revealed by a panel of monoclonal antibodies.
