ARG56406
anti-METTL3 antibody
anti-METTL3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes METTL3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | METTL3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human METTL3 |
| Conjugation | Un-conjugated |
| Alternate Names | Spo8; IME4; Methyltransferase-like protein 3; EC 2.1.1.62; N6-adenosine-methyltransferase 70 kDa subunit; M6A; MT-A70 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q86U44 Human N6-adenosine-methyltransferase 70 kDa subunit Swiss-port # Q8C3P7 Mouse N6-adenosine-methyltransferase subunit METTL3 |
|---|---|
| Gene Symbol | METTL3 |
| Gene Full Name | methyltransferase like 3 |
| Background | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| Function | N6-methyltransferase that methylates adenosine residues of some RNAs and acts as a regulator of the circadian clock, differentiation of embryonic stem cells and primary miRNA processing. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing, editing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop (By similarity). M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs (By similarity). M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). Mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8. [UniProt] |
| Calculated MW | 64 kDa |
Images (3) Click the Picture to Zoom In
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ARG56406 anti-METTL3 antibody WB image
Western blot: 30 µg of 293T, COS-7 and Mouse testis lysates stained with ARG56406 anti-METTL3 antibody at 1:500 dilution.
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ARG56406 anti-METTL3 antibody ICC/IF image
Immunofluorescence: C6 cells stained with ARG56406 anti-METTL3 antibody.
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ARG56406 anti-METTL3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG56406 anti-METTL3 antibody at 1:100 dilution.
Customer's Feedback
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Review for anti-METTL3 antibody
Application：WB
Sample：HepG2
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Transcriptomic analysis and laboratory experiments reveal potential critical genes and regulatory mechanisms in sepsis-associated acute kidney injury
WB / Mouse
