ARG44512

anti-METTL18 antibody

anti-METTL18 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes METTL18
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name METTL18
Antigen Species Human
Immunogen Human METTL18 recombinant protein
Conjugation Un-conjugated
Alternate Names METTL18; Methyltransferase 18, RPL3 N3(Tau)-Histidine; AsTP2; Methyltransferase Like 18 ; C1orf156; HPM1; Histidine Protein Methyltransferase 1 Homolog; Methyltransferase 18, RPL3 N3-Histidine; Methyltransferase-Like Protein 18; Arsenic-Transactivated Protein 2; MGC9084; Histidine Protein Methyltransferase 1; Chromosome 1 Open Reading Frame 156

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 92342 Human METTL18

Swiss-port # O95568 Human Histidine protein methyltransferase 1 homolog

Gene Symbol METTL18
Gene Full Name Methyltransferase 18, RPL3 N3(Tau)-Histidine
Background Enables heat shock protein binding activity and protein-L-histidine N-tele-methyltransferase activity. Involved in peptidyl-lysine monomethylation; regulation of gene expression; and regulation of ribosome biogenesis. Located in cytosol and nucleolus. Part of protein-containing complex.
Function Protein-L-histidine N-tele-methyltransferase that specifically monomethylates RPL3, thereby regulating translation elongation.
Cellular Localization Cytoplasm, Nucleus
Calculated MW 42 kDa
PTM Methylation, Phosphoprotein

Images (5) Click the Picture to Zoom In

  • ARG44512 anti-METTL18 antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG44512 anti-METTL18 antibody at 2 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44512 anti-METTL18 antibody at 5 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody WB image

    Western blot: A431 and PC-3 stained with ARG44512 anti-METTL18 antibody at 0.5 μg/mL dilution.

  • ARG44512 anti-METTL18 antibody FACS image

    Flow Cytometry: A431 stained with ARG44512 anti-METTL18 antibody at 1 μg/10^6 cells dilution.

  • ARG44512 anti-METTL18 antibody IHC-P image

    Immunohistochemistry: Human placenta stained with ARG44512 anti-METTL18 antibody at 2 μg/mL dilution.