ARG59873
anti-METTL14 antibody
anti-METTL14 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes METTL14 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | METTL14 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-210 of Human METTL14 (NP_066012.1). |
| Conjugation | Un-conjugated |
| Alternate Names | N6-adenosine-methyltransferase subunit METTL14; EC 2.1.1.62; Methyltransferase-like protein 14 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549 and Mouse brain | ||||||
| Observed Size | 62 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3UIK4 Mouse N6-adenosine-methyltransferase subunit METTL14 Swiss-port # Q9HCE5 Human N6-adenosine-methyltransferase subunit METTL14 |
|---|---|
| Gene Symbol | METTL14 |
| Gene Full Name | methyltransferase like 14 |
| Function | N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | Phosphorylation at Ser-399 is important for interaction with METTL3: phosphorylated Ser-399 forms a salt bridge with 'Arg-471' of METTL3. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG59873 anti-METTL14 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue stained with ARG59873 anti-METTL14 antibody at 1:100 dilution.
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ARG59873 anti-METTL14 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG59873 anti-METTL14 antibody at 1:1000 dilution.
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ARG59873 anti-METTL14 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG59873 anti-METTL14 antibody at 1:1000 dilution.
