ARG44189
anti-METAP2 antibody
anti-METAP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes METAP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | METAP2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human METAP2 |
| Conjugation | Un-conjugated |
| Protein Full Name | Methionine aminopeptidase 2 |
| Alternate Names | METAP2; Methionyl Aminopeptidase 2; MNPEP; MAP2; P67; Initiation Factor 2-Associated 67 KDa Glycoprotein Methionine Aminopeptidase 2; Peptidase M; P67eIF2; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Peptidase M 2; EC 3.4.11.18; P67EIF2; MetAP 2; MAP 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | METAP2 |
| Gene Full Name | Methionyl Aminopeptidase 2 |
| Background | The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. |
| Cellular Localization | Cytoplasm |
| Calculated MW | 53 kDa |
| PTM | Acetylation, Glycoprotein, Phosphoprotein |
Images (6) Click the Picture to Zoom In
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ARG44189 anti-METAP2 antibody IHC-P image
Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44189 anti-METAP2 antibody at 2 μg/mL dilution.
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ARG44189 anti-METAP2 antibody WB image
Western blot: HepG, MCF-7 and HeLa stained with ARG44189 anti-METAP2 antibody at 0.5 μg/mL dilution.
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ARG44189 anti-METAP2 antibody WB image
Western blot: Rat brain and PC-12 stained with ARG44189 anti-METAP2 antibody at 0.5 μg/mL dilution.
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ARG44189 anti-METAP2 antibody WB image
Western blot: Mouse pancrease and Mouse testis stained with ARG44189 anti-METAP2 antibody at 0.5 μg/mL dilution.
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ARG44189 anti-METAP2 antibody IHC-P image
Immunohistochemistry: Human tonsil stained with ARG44189 anti-METAP2 antibody at 2 μg/mL dilution.
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ARG44189 anti-METAP2 antibody WB image
Western blot: COS-7 stained with ARG44189 anti-METAP2 antibody at 0.5 μg/mL dilution.
