ARG57005

anti-MEMO1 antibody [1E9]

anti-MEMO1 antibody [1E9] for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [1E9] recognizes MEMO1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 1E9
Isotype IgG1, kappa
Target Name MEMO1
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-297 of Human MEMO1.
Conjugation Un-conjugated
Alternate Names Memo-1; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; C2orf4; MEMO; CGI-27; NS5ATP7; Hepatitis C virus NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Protein MEMO1; C21orf19-like protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
WB1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51072 Human MEMO1

Swiss-port # Q9Y316 Human Protein MEMO1

Gene Symbol MEMO1
Gene Full Name mediator of cell motility 1
Function May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration. [UniProt]
Calculated MW 34 kDa

Images (3) Click the Picture to Zoom In

  • ARG57005 anti-MEMO1 antibody [1E9] FACS image

    Flow Cytometry: U87MG cell line stained with ARG57005 anti-MEMO1 antibody [1E9] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.

  • ARG57005 anti-MEMO1 antibody [1E9] WB image

    Western blot: 40 µg of Jurkat cell lysate stained with ARG57005 anti-MEMO1 antibody [1E9] at 1:3000.

  • ARG57005 anti-MEMO1 antibody [1E9] WB image

    Western blot: 40 µg of 1) U-87 MG cell lysate, 2) Brain tissue lysate stained with ARG57005 anti-MEMO1 antibody [1E9] at 1:3000.