ARG57005
anti-MEMO1 antibody [1E9]
anti-MEMO1 antibody [1E9] for Flow cytometry,ICC/IF,Western blot and Human
Overview
Product Description | Mouse Monoclonal antibody [1E9] recognizes MEMO1 |
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Tested Reactivity | Hu |
Tested Application | FACS, ICC/IF, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | 1E9 |
Isotype | IgG1, kappa |
Target Name | MEMO1 |
Antigen Species | Human |
Immunogen | Recombinant fragment around aa. 1-297 of Human MEMO1. |
Conjugation | Un-conjugated |
Alternate Names | Memo-1; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; C2orf4; MEMO; CGI-27; NS5ATP7; Hepatitis C virus NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Protein MEMO1; C21orf19-like protein |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 10% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | MEMO1 |
Gene Full Name | mediator of cell motility 1 |
Function | May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration. [UniProt] |
Calculated MW | 34 kDa |
Images (3) Click the Picture to Zoom In
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ARG57005 anti-MEMO1 antibody [1E9] FACS image
Flow Cytometry: U87MG cell line stained with ARG57005 anti-MEMO1 antibody [1E9] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57005 anti-MEMO1 antibody [1E9] WB image
Western blot: 40 µg of Jurkat cell lysate stained with ARG57005 anti-MEMO1 antibody [1E9] at 1:3000.
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ARG57005 anti-MEMO1 antibody [1E9] WB image
Western blot: 40 µg of 1) U-87 MG cell lysate, 2) Brain tissue lysate stained with ARG57005 anti-MEMO1 antibody [1E9] at 1:3000.