ARG63353
anti-MEL18 / PCGF2 antibody
anti-MEL18 / PCGF2 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MEL18 / PCGF2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MEL18 / PCGF2 |
| Antigen Species | Human |
| Immunogen | C-KMTVNGAPVPPLT |
| Conjugation | Un-conjugated |
| Alternate Names | RING finger protein 110; Polycomb group RING finger protein 2; ZNF144; MEL-18; RNF110; Zinc finger protein 144; DNA-binding protein Mel-18 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35227 Human Polycomb group RING finger protein 2 |
|---|---|
| Background | The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 38 kDa |
| PTM | Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG63353 anti-MEL18 / PCGF2 antibody WB image
Western Blot: Human Lung lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63353 anti-MEL18 antibody at 1 µg/ml dilution.
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ARG63353 anti-MEL18 / PCGF2 antibody WB image
Western blot: 30 µg of A549 (A) and PD19 (B) cell lysates (in RIPA buffer) stained with ARG63353 anti-MEL18 / PCGF2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
