ARG51108

anti-MEK3 / MKK3 antibody

anti-MEK3 / MKK3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MEK3 / MKK3
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MEK3 / MKK3
Antigen Species Human
Immunogen Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3 / MEK3.
Conjugation Un-conjugated
Alternate Names SAPK kinase 2; MEK 3; MAPKK 3; Stress-activated protein kinase kinase 2; PRKMK3; EC 2.7.12.2; MAPK/ERK kinase 3; MEK3; MAPKK3; SAPKK2; SAPKK-2; MAP kinase kinase 3; MKK3; Dual specificity mitogen-activated protein kinase kinase 3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5606 Human MAP2K3

Swiss-port # P46734 Human Dual specificity mitogen-activated protein kinase kinase 3

Gene Symbol MAP2K3
Gene Full Name mitogen-activated protein kinase kinase 3
Background MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
Function Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. [UniProt]
Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 39 kDa
PTM Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118).
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Images (3) Click the Picture to Zoom In

  • ARG51108 anti-MEK3 / MKK3 antibody ICC/IF image

    Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51108 anti-MEK3 / MKK3 antibody.

  • ARG51108 anti-MEK3 / MKK3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51108 anti-MEK3 / MKK3 antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51108 anti-MEK3 / MKK3 antibody WB image

    Western blot: Extracts from MDA cells stained with ARG51108 anti-MEK3 / MKK3 antibody.