ARG59444
anti-MED9 antibody
anti-MED9 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED9 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED9 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A55-E146 of Human MED9. |
| Conjugation | Un-conjugated |
| Alternate Names | Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED25 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8VCS6 Mouse Mediator of RNA polymerase II transcription subunit 9 Swiss-port # Q9NWA0 Human Mediator of RNA polymerase II transcription subunit 9 |
|---|---|
| Gene Symbol | MED9 |
| Gene Full Name | mediator complex subunit 9 |
| Background | The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 16 kDa |
Images (8) Click the Picture to Zoom In
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ARG59444 anti-MED9 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59444 anti-MED9 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59444 anti-MED9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was stained with ARG59444 anti-MED9 antibody at 1 µg/ml, overnight at 4°C.
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ARG59444 anti-MED9 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat heart, Rat kidney, Mouse brain, Mouse heart and Mouse kidney tissue lysates stained with ARG59444 anti-MED9 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59444 anti-MED9 antibody FACS image
Flow Cytometry: MCF-7 cells were blocked with 10% normal goat serum and then stained with ARG59444 anti-MED9 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59444 anti-MED9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59444 anti-MED9 antibody at 1 µg/ml, overnight at 4°C.
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ARG59444 anti-MED9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59444 anti-MED9 antibody at 1 µg/ml, overnight at 4°C.
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ARG59444 anti-MED9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59444 anti-MED9 antibody at 1 µg/ml, overnight at 4°C.
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ARG59444 anti-MED9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59444 anti-MED9 antibody at 1 µg/ml, overnight at 4°C.
