ARG44190

anti-MED29 antibody

anti-MED29 antibody for ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MED29
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MED29
Antigen Species Human
Immunogen Recombinant protein of Human MED29
Conjugation Un-conjugated
Protein Full Name Mediator of RNA polymerase II transcription subunit 29
Alternate Names MED29; Mediator Complex Subunit 29; MED2; IXL; Mediator Of RNA Polymerase II Transcription Subunit 29; Intersex-Like Protein; DKFZp434H247; Intersex-Like (Drosophila)

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55588 Human MED29

Swiss-port # Q9NX70 Human Mediator of RNA polymerase II transcription subunit 29

Gene Symbol MED29
Gene Full Name Mediator Complex Subunit 29
Background MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors.
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Localization Nucleus
Calculated MW 21 kDa
PTM Acetylation

Images (2) Click the Picture to Zoom In

  • ARG44190 anti-MED29 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG44190 anti-MED29 antibody at 5 μg/mL dilution.

  • ARG44190 anti-MED29 antibody WB image

    Western blot: MCF-7 and 293T stained with ARG44190 anti-MED29 antibody at 0.5 μg/mL dilution.