ARG44122
anti-MED26 antibody
anti-MED26 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal recognizes MED26 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED26 |
| Antigen Species | Human |
| Immunogen | Human MED26 recombinant protein (Position: N24-D600). |
| Conjugation | Un-conjugated |
| Alternate Names | MED26; Mediator Complex Subunit 26; CRSP70; CRSP7; Cofactor Required For Sp1 Transcriptional Activation, Subunit 7, 70kDa ; Cofactor Required For Sp1 Transcriptional Activation Subunit 7; Mediator Of RNA Polymerase II Transcription Subunit 26; Activator-Recruited Cofactor 70 KDa Component; Transcriptional Coactivator CRSP70; CRSP Complex Subunit 7; ARC70; Cofactor Required For Sp1 Transcriptional Activation, Subunit 7 (70kD) |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95402 Human Mediator of RNA polymerase II transcription subunit 26 |
|---|---|
| Gene Symbol | MED26 |
| Gene Full Name | Mediator Complex Subunit 26 |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. |
| Cellular Localization | Nucleus |
| Calculated MW | 65 kDa |
| PTM | Phosphoprotein |
Images (4) Click the Picture to Zoom In
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ARG44122 anti-MED26 antibody IHC-P image
Immunohistochemistry: Human parotid acinar cell carcinoma stained with ARG44122 anti-MED26 antibody at 2 μg/ml dilution.
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ARG44122 anti-MED26 antibody WB image
Western blot: 293T and HepG2 stained with ARG44122 anti-MED26 antibody at 0.5 μg/ml dilution.
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ARG44122 anti-MED26 antibody FACS image
Flow Cytometry: 293T stained with ARG44122 anti-MED26 antibody at 1 µg/10^6 cells dilution.
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ARG44122 anti-MED26 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG44122 anti-MED26 antibody at 2 μg/ml dilution.
