ARG64854

anti-MED15 / PCQAP antibody

anti-MED15 / PCQAP antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes MED15 / PCQAP
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application WB
Specificity This antibody is expected to recognize both reported isoforms (NP_001003891.1; NP_056973.2).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MED15 / PCQAP
Antigen Species Human
Immunogen C-PPQSQTQPLVSQAQ
Conjugation Un-conjugated
Alternate Names Activator-recruited cofactor 105 kDa component; PC2 glutamine/Q-rich-associated protein; Mediator complex subunit 15; TPA-inducible gene 1 protein; ARC105; Positive cofactor 2 glutamine/Q-rich-associated protein; CTG7A; Trinucleotide repeat-containing gene 7 protein; Mediator of RNA polymerase II transcription subunit 15; CTG repeat protein 7a; CAG7A; TIG-1; TIG1; PCQAP; TNRC7

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51586 Human MED15

Swiss-port # Q96RN5 Human Mediator of RNA polymerase II transcription subunit 15

Gene Symbol MED15
Gene Full Name mediator complex subunit 15
Background The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 87 kDa
PTM Ubiquitinated by TRIM11, leading to proteasomal degradation.

Images (1) Click the Picture to Zoom In

  • ARG64854 anti-MED15 / PCQAP antibody WB image

    Western blot: 35 µg of Human lung lysate (in RIPA buffer) stained with ARG64854 anti-MED15 / PCQAP antibody at 1 µg/ml dilution.