ARG55387
anti-MED12 antibody
anti-MED12 antibody for Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED12 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED12 |
| Antigen Species | Mouse |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1991-2025 (C-terminus) of Mouse MED12. |
| Conjugation | Un-conjugated |
| Alternate Names | ARC240; OKS; TNRC11; Thyroid hormone receptor-associated protein complex 230 kDa component; TRAP230; OPA-containing protein; OPA1; Mediator complex subunit 12; MED12S; FGS1; CAGH45; Mediator of RNA polymerase II transcription subunit 12; Activator-recruited cofactor 240 kDa component; Trinucleotide repeat-containing gene 11 protein; OHDOX; HOPA; CAG repeat protein 45; Trap230 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # A2AGH6 Mouse Mediator of RNA polymerase II transcription subunit 12 Swiss-port # Q93074 Human Mediator of RNA polymerase II transcription subunit 12 |
|---|---|
| Gene Symbol | Med12 |
| Gene Full Name | mediator complex subunit 12 |
| Background | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity). [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 243 kDa |
Images (1) Click the Picture to Zoom In
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ARG55387 anti-MED12 antibody WB image
Western blot: 20 µg of HeLa whole cell lysate stained with ARG55387 anti-MED12 antibody at 1:2000 dilution.
