ARG43423
anti-MED1 / TRAP220 antibody
anti-MED1 / TRAP220 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED1 / TRAP220 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED1 / TRAP220 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 664-720 of Human MED1 / TRAP220. |
| Conjugation | Un-conjugated |
| Alternate Names | TR-interacting protein 2; TRIP-2; Mediator of RNA polymerase II transcription subunit 1; DRIP230; PPAR-binding protein; Vitamin D receptor-interacting protein complex component DRIP205; Peroxisome proliferator-activated receptor-binding protein; Trap220; DRIP205; CRSP1; TRAP220; PBP; p53 regulatory protein RB18A; CRSP200; Mediator complex subunit 1; Thyroid receptor-interacting protein 2; PPARBP; TRIP2; PPARGBP; ARC205; Thyroid hormone receptor-associated protein complex 220 kDa component; RB18A; Activator-recruited cofactor 205 kDa component |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549, HEK293T, PC12 and CT26 | ||||||
| Observed Size | ~ 250 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.2), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15648 Human Mediator of RNA polymerase II transcription subunit 1 Swiss-port # Q925J9 Mouse Mediator of RNA polymerase II transcription subunit 1 |
|---|---|
| Gene Symbol | MED1 |
| Gene Full Name | mediator complex subunit 1 |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). [UniProt] |
| Cellular Localization | Nucleus. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3. [UniProt] |
| Calculated MW | 168 kDa |
| PTM | Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG43423 anti-MED1 / TRAP220 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG43423 anti-MED1 / TRAP220 antibody at 1:100 dilution.
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ARG43423 anti-MED1 / TRAP220 antibody WB image
Western blot: 40 µg of A549, HEK293T, PC12 and CT26 whole cell lysates stained with ARG43423 anti-MED1 / TRAP220 antibody at 1:500 dilution.
