ARG62541
anti-MCP5 / Mast cell chymase antibody [CC1]
anti-MCP5 / Mast cell chymase antibody [CC1] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Pig
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [CC1] recognizes MCP5 / Mast cell chymase |
|---|---|
| Tested Reactivity | Hu, Rat, Pig |
| Tested Application | FACS, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CC1 |
| Isotype | IgG1 |
| Target Name | MCP5 / Mast cell chymase |
| Antigen Species | Human |
| Immunogen | Purified human skin Chymase |
| Conjugation | Un-conjugated |
| Alternate Names | Chymase; Alpha-chymase; Mast cell protease I; chymase; CYH; EC 3.4.21.39; MCT1 |
Application Instructions
| Application Note | FACS: 1-2µg for 106 cells IHC: 1/100-1/200 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS, 1% BSA and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CMA1 |
| Gene Full Name | chymase 1, mast cell |
| Background | This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015] |
| Function | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. [UniProt] |
| Cellular Localization | Secreted, Cytoplasmic granule |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 27 kDa |
Clone References
Stress management affects outcomes in the pathophysiology of an endometriosis model.
Altered immunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium.
IHC-P / Human
Chymase mediates injury and mitochondrial damage in cardiomyocytes during acute ischemia/reperfusion in the dog.
ICC/IF / Mouse
Mast cells in COPD airways: relationship to bronchodilator responsiveness and angiogenesis.
IHC-P / Human
Impact of acute myocardial ischemia reperfusion on the tissue and blood-borne renin-angiotensin system.
WB / Pig
Cannabinoids reduce granuloma-associated angiogenesis in rats by controlling transcription and expression of mast cell protease-5.
WB / Rat
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
IHC / Rat
