ARG66020
anti-MCP5 / Mast cell chymase antibody (Biotin)
anti-MCP5 / Mast cell chymase antibody (Biotin) for ELISA,Western blot and Mouse
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes MCP5 / Mast cell chymase |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MCP5 / Mast cell chymase |
| Antigen Species | Mouse |
| Immunogen | E. coli derived recombinant Mouse MCP5. (GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG) |
| Conjugation | Biotin |
| Alternate Names | Chymase; Alpha-chymase; Mast cell protease I; chymase; CYH; EC 3.4.21.39; MCT1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Cma1 |
| Gene Full Name | chymase 1, mast cell |
| Background | This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015] |
| Function | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. [UniProt] |
| Calculated MW | 27 kDa |
Images (4) Click the Picture to Zoom In
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ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin), under non-reducing conditions.
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ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) standard curve image
Direct ELISA: ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin), under reducing conditions.
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ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) standard curve image
Sandwich ELISA: ARG66020 anti-MCP5 / Mast cell chymase antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66019 anti-MCP5 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
