ARG66019
anti-MCP5 / Mast cell chymase antibody
anti-MCP5 / Mast cell chymase antibody for ELISA,Neutralizing,Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MCP5 / Mast cell chymase |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MCP5 / Mast cell chymase |
| Antigen Species | Mouse |
| Immunogen | E. coli derived recombinant Mouse MCP5. (GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG) |
| Conjugation | Un-conjugated |
| Alternate Names | Chymase; Alpha-chymase; Mast cell protease I; chymase; CYH; EC 3.4.21.39; MCT1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Cma1 |
| Gene Full Name | chymase 1, mast cell |
| Background | This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015] |
| Function | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. [UniProt] |
| Calculated MW | 27 kDa |
Images (3) Click the Picture to Zoom In
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ARG66019 anti-MCP5 / Mast cell chymase antibody WB image
Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66019 anti-MCP5 / Mast cell chymase antibody, under reducing conditions.
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ARG66019 anti-MCP5 / Mast cell chymase antibody standard curve image
Sandwich ELISA: ARG66019 anti-MCP5 / Mast cell chymase antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66020 anti-MCP5 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66019 anti-MCP5 / Mast cell chymase antibody WB image
Western blot: 250 - 0.24 ng of Mouse MCP-5 stained with ARG66019 anti-MCP5 / Mast cell chymase antibody, under non-reducing conditions.
