ARG56702

anti-MCP3 antibody

anti-MCP3 antibody for ELISA,Neutralizing,Western blot and Mouse

Overview

Product Description Goat Polyclonal antibody recognizes MCP3
Tested Reactivity Ms
Tested Application ELISA, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MCP3
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse MCP-3 (CCL7).
(QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP)
Conjugation Un-conjugated
Alternate Names MCP3; NC28; Monocyte chemotactic protein 3; MARC; Small-inducible cytokine A7; FIC; C-C motif chemokine 7; Monocyte chemoattractant protein 3; MCP-3; SCYA7; SCYA6

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56811 as a detection antibody
Neut4.0 - 6.0 µg/ml (To yield [ND50] of the biological activity of Murine MCP - 3 (100.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20306 Mouse CCL7

Swiss-port # Q03366 Mouse C-C motif chemokine 7

Gene Symbol Ccl7
Gene Full Name chemokine (C-C motif) ligand 7
Background This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]
Function Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. [UniProt]
Calculated MW 11 kDa
PTM O-glycosylated.

Images (3) Click the Picture to Zoom In

  • ARG56702 anti-MCP3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse MCP-3 stained with ARG56702 anti-MCP3 antibody, under reducing conditions.

  • ARG56702 anti-MCP3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse MCP-3 stained with ARG56702 anti-MCP3 antibody, under non-reducing conditions.

  • ARG56702 anti-MCP3 antibody standard curve image

    Sandwich ELISA: ARG56702 anti-MCP3 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56811 anti-MCP3 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.