Overview

Product Description	Rabbit Polyclonal antibody recognizes MCM3
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MCM3
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human MCM3.
Conjugation	Un-conjugated
Alternate Names	DNA polymerase alpha holoenzyme-associated protein P1; RLF subunit beta; P1.h; HCC5; DNA replication licensing factor MCM3; p102; EC 3.6.4.12; P1-MCM3; RLFB

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 17215 Mouse MCM3 GeneID: 4172 Human MCM3 Swiss-port # P25205 Human DNA replication licensing factor MCM3 Swiss-port # P25206 Mouse DNA replication licensing factor MCM3
Gene Symbol	MCM3
Gene Full Name	minichromosome maintenance complex component 3
Background	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Function	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. [UniProt]
Cellular Localization	Nucleus. [UniProt]
Calculated MW	91 kDa
PTM	O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt]

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