ARG55091
anti-MCM-BP antibody
anti-MCM-BP antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MCM-BP |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Bov |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MCM-BP |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 143-171 (N-terminus) of Human MCM-BP. |
| Conjugation | Un-conjugated |
| Alternate Names | C10orf119; MCM-binding protein; Mini-chromosome maintenance complex-binding protein; MCM-BP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MDA-MB-231 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BTE3 Human Mini-chromosome maintenance complex-binding protein |
|---|---|
| Gene Symbol | MCMBP |
| Gene Full Name | minichromosome maintenance complex binding protein |
| Background | This gene encodes a protein which is a component of the hexameric minichromosome maintenance (MCM) complex which regulates initiation and elongation of DNA. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion. [UniProt] |
| Cellular Localization | Nucleus. Note=Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 73 kDa |
Images (3) Click the Picture to Zoom In
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ARG55091 anti-MCM-BP antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cervix carcinoma tissue stained with ARG55091 anti-MCM-BP antibody.
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ARG55091 anti-MCM-BP antibody WB image
Western blot: 35 µg of MDA-MB-231 cell lysate stained with ARG55091 anti-MCM-BP antibody.
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ARG55091 anti-MCM-BP antibody FACS image
Flow Cytometry: MDA-MB231 cells stained with ARG55091 anti-MCM-BP antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
