ARG44226

anti-MCCC1 antibody

anti-MCCC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MCCC1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MCCC1
Antigen Species Human
Immunogen Recombinant protein of Human MCCC1
Conjugation Un-conjugated
Protein Full Name Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Alternate Names MCCC1; Methylcrotonyl-CoA Carboxylase Subunit 1; MCCA; Methylcrotonoyl-CoA Carboxylase Subunit Alpha, Mitochondrial; 3-Methylcrotonyl-CoA Carboxylase Biotin-Containing Subunit; 3-Methylcrotonyl-CoA:Carbon Dioxide Ligase Subunit Alpha; Methylcrotonoyl-Coenzyme A Carboxylase 1 (Alpha); Methylcrotonoyl-CoA Carboxylase Alpha; 3-Methylcrotonyl-CoA Carboxylase 1; MCCase Subunit Alpha; EC 6.4.1.4; 3-Methylcrotonyl-CoA Carboxylase Biotin Containing Subunit; Methylcrotonoyl-CoA Carboxylase 1 (Alpha); Methylcrotonoyl-CoA Carboxylase 1; MCCCalpha; EC 6.4.1; MCCCα; MCC-B; MCCCA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 294972 Rat MCCC1

GeneID: 56922 Human MCCC1

GeneID: 72039 Mouse MCCC1

Gene Symbol MCCC1
Gene Full Name Methylcrotonyl-Coa Carboxylase Subunit 1
Background This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
Function Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
Cellular Localization Mitochondrion
Calculated MW 80 kDa
PTM Acetylation

Images (6) Click the Picture to Zoom In

  • ARG44226 anti-MCCC1 antibody IHC-P image

    Immunohistochemistry: Human thyroid cancer stained with ARG44226 anti-MCCC1 antibody at 2 μg/mL dilution.

  • ARG44226 anti-MCCC1 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44226 anti-MCCC1 antibody at 5 μg/mL dilution.

  • ARG44226 anti-MCCC1 antibody WB image

    Western blot: SIHA and RT4 stained with ARG44226 anti-MCCC1 antibody at 0.5 μg/mL dilution.

  • ARG44226 anti-MCCC1 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG44226 anti-MCCC1 antibody at 2 μg/mL dilution.

  • ARG44226 anti-MCCC1 antibody WB image

    Western blot: Rat liver stained with ARG44226 anti-MCCC1 antibody at 0.5 μg/mL dilution.

  • ARG44226 anti-MCCC1 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG44226 anti-MCCC1 antibody at 2 μg/mL dilution.