ARG64809
anti-MBNL1 antibody
anti-MBNL1 antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MBNL1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize the reported isoforms (NP_066368.2; NP_997175.1; NP_997176.1; NP_997177.1; NP_997178.1; NP_997179.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MBNL1 |
| Antigen Species | Human |
| Immunogen | C-SAEHLTSHKYVTQ |
| Conjugation | Un-conjugated |
| Alternate Names | Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; MBNL; EXP |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MBNL1 |
| Gene Full Name | muscleblind-like splicing regulator 1 |
| Function | Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 42 kDa |
Images (1) Click the Picture to Zoom In
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ARG64809 anti-MBNL1 antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG64809 anti-MBNL1 antibody at 1 µg/ml dilution.
