ARG42933
anti-MBD4 / MED1 antibody
anti-MBD4 / MED1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MBD4 / MED1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MBD4 / MED1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A16-S580 of Human MBD4 / MED1. |
| Conjugation | Un-conjugated |
| Alternate Names | Methyl-CpG-binding endonuclease 1; MED1; Methyl-CpG-binding domain protein 4; Mismatch-specific DNA N-glycosylase; EC 3.2.2.-; Methyl-CpG-binding protein MBD4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95243 Human Methyl-CpG-binding domain protein 4 Swiss-port # Q9Z2D7 Mouse Methyl-CpG-binding domain protein 4 |
|---|---|
| Gene Symbol | MBD4 |
| Gene Full Name | methyl-CpG binding domain 4 DNA glycosylase |
| Background | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
| Function | Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 66 kDa |
Images (4) Click the Picture to Zoom In
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ARG42933 anti-MBD4 / MED1 antibody ICC/IF image
Immunofluorescence: HeLa cells were blocked with 10% goat serum and then stained with ARG42933 anti-MBD4 / MED1 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42933 anti-MBD4 / MED1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42933 anti-MBD4 / MED1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42933 anti-MBD4 / MED1 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG42933 anti-MBD4 / MED1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42933 anti-MBD4 / MED1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42933 anti-MBD4 / MED1 antibody at 1 µg/ml dilution, overnight at 4°C.
