Overview

Product Description	Rabbit Polyclonal antibody recognizes MBD1
Tested Reactivity	Hu
Tested Application	ICC/IF, IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MBD1
Antigen Species	Human
Immunogen	Synthetic peptide of Human MBD1.
Conjugation	Un-conjugated
Alternate Names	RFT; PCM1; CXXC-type zinc finger protein 3; CXXC3; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 IP 1:20 WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 77 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4152 Human MBD1 Swiss-port # Q9UIS9 Human Methyl-CpG-binding domain protein 1
Gene Symbol	MBD1
Gene Full Name	methyl-CpG binding domain protein 1
Background	The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]
Function	Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. [UniProt]
Cellular Localization	Nucleus. Nucleus matrix. Nucleus speckle. Chromosome. Note=Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. [UniProt]
Calculated MW	67 kDa
PTM	Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. [UniProt]

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