ARG40405

anti-MAT1A antibody

anti-MAT1A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MAT1A
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Gpig, Hrs, Rb, Zfsh
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MAT1A
Antigen Species Human
Immunogen Synthetic peptide around the N-terminal region of Human MAT1A. (within the following region: TSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE)
Conjugation Un-conjugated
Alternate Names SAMS1; MAT 1; MAT; Methionine adenosyltransferase I/III; MAT-I/III; Methionine adenosyltransferase 1; MATA1; AdoMet synthase 1; EC 2.5.1.6; SAMS; S-adenosylmethionine synthase isoform type-1

Application Instructions

Predict Reactivity Note Predicted Homology Based On Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Application Suggestion
Tested Application Dilution
IHC-P4 - 8 µg/ml
WB1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size 48 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% (w/v) Sodium azide and 2% Sucrose.
Preservative 0.09% (w/v) Sodium azide
Stabilizer 2% Sucrose
Concentration Batch dependent: 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4143 Human MAT1A

Swiss-port # Q00266 Human S-adenosylmethionine synthase isoform type-1

Gene Symbol MAT1A
Gene Full Name methionine adenosyltransferase I, alpha
Background This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]
Function Catalyzes the formation of S-adenosylmethionine from methionine and ATP. [UniProt]
Calculated MW 44 kDa
PTM S-nitrosylation of Cys-120 inactivates the enzyme.

An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG40405 anti-MAT1A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney (Epithelial cells of renal tubule) stained with ARG40405 anti-MAT1A antibody at 4 - 8 µg/ml dilution.

  • ARG40405 anti-MAT1A antibody WB image

    Western blot: Jurkat cell lysate stained with ARG40405 anti-MAT1A antibody at 1.25 µg/ml dilution.