ARG64487
anti-MARK2 / PAR1 antibody
anti-MARK2 / PAR1 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MARK2 / PAR1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise isoform c (NP_004945.4), isoform e (NP_001156768.1) and isoform f (NP_001156769.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MARK2 / PAR1 |
| Antigen Species | Human |
| Immunogen | C-QNGKDSTAPQR |
| Conjugation | Un-conjugated |
| Alternate Names | PAR1 homolog b; Serine/threonine-protein kinase MARK2; EC 2.7.11.26; EMK-1; MAP/microtubule affinity-regulating kinase 2; Par1b; EMK1; Par-1b; PAR1 homolog; EC 2.7.11.1; ELKL motif kinase 1; PAR-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7KZI7 Human Serine/threonine-protein kinase MARK2 |
|---|---|
| Background | This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 88 kDa |
| PTM | Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm. |
Images (1) Click the Picture to Zoom In
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ARG64487 anti-MARK2 / PAR1 antibody WB image
Western blot: Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) stained with ARG64487 anti-MARK2 / PAR1 antibody at 0.3 µg/ml dilution.
